Journal:

Article Title: Transcriptional and Posttranscriptional Inhibition of Lysyl Oxidase Expression by Cigarette Smoke Condensate in Cultured Rat Fetal Lung Fibroblasts

doi: 10.1093/toxsci/kfi212

Figure Lengend Snippet: RFL6 cells were co-transfected with LO promoter-reporter plasmids and pSV-β-galactosidase (β-gal) vectors. After 6 h posttransfection, cells were washed, incubated in 10% FBS/DMEM for an additional 18 h period, washed again, and exposed to CSC at indicated doses for 24 h. Luciferase and β-gal activities in control and treated cells were measured according to manufacturer’s instructions. LO-promoter activities in transfected cells were normalized to β-gal activities and expressed as cpm/OD β-gal (100% luciferase activity in the control = 3,528 ± 312 cpm/OD β-gal). ** p < 0.01 vs. control for cells treated with 80 and 120 μg/ml of CSC .

Article Snippet: Rat fetal lung fibroblasts (RFL6) obtained from ATCC were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) as previously described ( Chen et al ., 2005 ).

Techniques: Transfection, Incubation, Luciferase, Control, Activity Assay

Journal:

Article Title: Induction of Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells by Transforming Growth Factor-?1

doi:

Figure Lengend Snippet: Effects of TGF-β1 ± TNF-α on cell morphology and expression of α-SMA in RLE-6TN cells. Immunoreactivity for α-SMA (green) was assessed by immunofluorescence on day 6, and accompanying phase images of cell morphology were obtained. Expression of α-SMA and assumption of a fibroblast-like morphology was induced in monolayers exposed to TGF-β1 and was seen in 100% of cells in cultures treated with TGF-β1 and TNF-α. A: Phase image of monolayer in media only. B: Phase image of monolayer in media + TGF-β1. C: Phase image of monolayer in media + TGF-β1 + TNF-α. D and G: Monolayer in media only reacted with anti-α-SMA mAb. E and H: Monolayer in media + TGF-β1 reacted with anti-α-SMA mAb. F and I: Monolayer in media + TGF-β1 + TNF-α reacted with anti-α-SMA mAb. Red staining represents propidium iodide-stained nuclei. Photographs are representative of >12 cultures from more than three separate experiments. Original magnification, ×400 for A through F; ×100 for G through I.

Article Snippet: 50–52 Consistent with this, control experiments using primary rat lung fibroblasts (Cell Applications, San Diego, CA) and a rat lung fibroblast cell line (RFL-6; American Type Culture Collection, Manassas, VA) showed that, in culture under the conditions described above, fibroblasts were eliminated within 24 hours (data not shown).

Techniques: Expressing, Immunofluorescence, Staining

Journal:

Article Title: Induction of Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells by Transforming Growth Factor-?1

doi:

Figure Lengend Snippet: Colocalization of α-SMA and TTF-1 in primary AECs during epithelial-mesenchymal transition. Immunoreactivity for α-SMA (green) and TTF-1 (red) was assessed on days 6, 8, and 10 of primary culture of AECs in MDSF + TGF-β1 + TNF-α. On day 6, individual AECs were identified that co-express nuclear TTF-1 and cytoplasmic α-SMA. Expression of α-SMA increased gradually over time in culture (A to C, D to F) and paralleled a concomitant decrease in expression of TTF-1 (A to C, G to I) along with a transition from an epithelial to a fibroblast-like morphology. Photographs are representative of >12 monolayers from more than three separate experiments. Original magnification, ×600.

Article Snippet: 50–52 Consistent with this, control experiments using primary rat lung fibroblasts (Cell Applications, San Diego, CA) and a rat lung fibroblast cell line (RFL-6; American Type Culture Collection, Manassas, VA) showed that, in culture under the conditions described above, fibroblasts were eliminated within 24 hours (data not shown).

Techniques: Expressing

Journal: International journal of cancer

Article Title: Antiangiogenic activity of endostatin inhibits C6 glioma growth.

doi: 10.1002/ijc.10115

Figure Lengend Snippet: FIGURE 3 – The antiproliferative effect of endogenously expressed endostatin on bFGF-stimulated BAECs. BAECs were cultured in standard medium or medium supplemented with bFGF, or bFGF and CM (1:3 final concentration) from C6Endo-E3 cells, C6Endo-E6 cells or parental C6 glioma cells. After 72 hr of culture, the antiproliferative effect of each CM was determined using a colorimetric assay (see Material and Methods) and proliferation measured at A490nm.

Article Snippet: Cell lines and culture conditions Rat C6 glioma cells (CCL 107:ATCC, ) were routinely cultured in RPMI 1640/5% new calf serum (NCS; heat inactivated) and 2 mM L-glutamine in a humidified atmosphere of 5% CO2.

Techniques: Cell Culture, Concentration Assay, Colorimetric Assay

Journal: International journal of cancer

Article Title: Antiangiogenic activity of endostatin inhibits C6 glioma growth.

doi: 10.1002/ijc.10115

Figure Lengend Snippet: FIGURE 4 – The growth rate of s.c. tumors implanted in athymic (nu/nu) mice. Tumor volumes were determined by tridimensional caliper measurements performed every second day throughout the experiment. Tumor volumes are presented as the mean mm3 stan- dard error (SE) of 5 mice per cell line in 2 experiments. Statistical significance was calculated at day 20 postimplantation (p 0.04). Tumors in mice implanted with C6Endo-E6 cells were also measured at day 22 postimplantation. Mice implanted with parental C6 cells or C6Endo-E3 cells were not continued to day 22 due to the large size of their tumors. C6, parental C6 glioma cells; E3, C6Endo-E3 cells; E6, C6Endo-E6 cells.

Article Snippet: Cell lines and culture conditions Rat C6 glioma cells (CCL 107:ATCC, ) were routinely cultured in RPMI 1640/5% new calf serum (NCS; heat inactivated) and 2 mM L-glutamine in a humidified atmosphere of 5% CO2.

Techniques: